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HMGCLL1 Polyclonal antibody

HMGCLL1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 17206-1-AP

Synonyms

HMGCLL1

Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using HMGCLL1 Polyclonal antibody (17206-1-AP)

    WB analysis of SKOV-3 using 17206-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 17206-1-AP (HMGCLL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HMGCLL1 Polyclonal antibody (17206-1-AP)

    IHC staining of human liver cancer using 17206-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17206-1-AP (HMGCLL1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HMGCLL1 Polyclonal antibody (17206-1-AP)

    IHC staining of human liver cancer using 17206-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17206-1-AP (HMGCLL1 antibody) at dilution of 1:200 (under 10x lens).

Tested Applications

Positive WB detected inSKOV-3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17206-1-AP is HMGCLL1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMGCLL1 fusion protein Ag10985
Full Name 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase-like 1
Calculated Molecular Weight 340 aa, 36 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC024194
Gene Symbol HMGCLL1
Gene ID (NCBI) 54511
RRIDAB_2878362
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HMGCLL1 (3-hydroxymethyl-3-methylglutaryl-CoA lyase-like protein 1), also named as er-cHL, is a close homolog of mitochondrial HMG-CoA lyase (mHL). HMGCLL1 catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, which is a key step in ketogenesis (PMID: 22847177, 22865860). HMGCLL1 has some isoforms with the molecular mass of 32-39 kDa and 6 kDa.

Protocols

Product Specific Protocols
WB protocol for HMGCLL1 antibody 17206-1-APDownload protocol
IHC protocol for HMGCLL1 antibody 17206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols