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KD/KO Validated

HN1 Polyclonal antibody

Name: HN1 Polyclonal antibody
Brand: Proteintech
SKU: 14914-1-AP

HN1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14914-1-AP

Synonyms

Androgen regulated protein 2, ARM2, HN1, HN1A

Validation Data Gallery

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AllWBIPIHC

WB analysis of HeLa using 14914-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 14914-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14914-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 14914-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 14914-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 14914-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 14914-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of LNCaP using 14914-1-AP
LNCaP cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 14914-1-AP
IP result of anti-HN1 (IP:14914-1-AP, 4ug; Detection:14914-1-AP 1:1000) with MCF-7 cells lysate 3200ug.
IHC staining of human ovary tumor using 14914-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 14914-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14914-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14914-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 14914-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, human brain tissue, human testis tissue, HepG2 cells, A431 cells, LNCaP cells, mouse testis tissue, mouse colon tissue
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human ovary tumor tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 5 publications below
IHC	See 2 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

14914-1-AP targets HN1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HN1 fusion protein Ag6712
Full Name	hematological and neurological expressed 1
Calculated Molecular Weight	11 kDa, 16 kDa, 19 kDa
Observed Molecular Weight	23-30 kDa
GenBank Accession Number	BC001420
Gene Symbol	HN1
Gene ID (NCBI)	51155
RRID	AB_10863118
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HN1 (hematopoietic- and neurologic-expressed sequence 1) is a highly conserved protein that is expressed in developing and regenerating tissues. Overexpression of HN1 has been reported in various tumors like glioma and breast cancer. Hn1 has also been reported as a marker for human ovarian carcinoma and can distinguish epithelial ovarian carcinoma cells from normal ovarian surface epithelial cells. This collective information suggests that HN1 is involved in processes associated with cell proliferation, repair and/or growth.

Protocols

Product Specific Protocols
WB protocol for HN1 antibody 14914-1-AP	Download protocol
IHC protocol for HN1 antibody 14914-1-AP	Download protocol
IP protocol for HN1 antibody 14914-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,CoIP	Cancer Lett HN1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by interacting with STMN1. Authors - Zongfu Pan KO Validated View Article
human	WB,IHC	Kaohsiung J Med Sci Increased expression of hematological and neurological expressed 1 (HN1) is associated with a poor prognosis of hepatocellular carcinoma and its knockdown inhibits cell growth and migration partly by down-regulation of c-Met. Authors - Jia-Jie Chen KD Validated View Article
human	WB	Chem Biol Drug Des Matrine exerts an anti-tumor effect via regulating HN1 in triple breast cancer both in vitro and in vivo Authors - Qiusheng Guo View Article
human	WB,IF	Cancer Lett Epigenetic inhibition of CTCF by HN1 promotes dedifferentiation and stemness of anaplastic thyroid cancer Authors - Zongfu Pan View Article
human	WB,IHC	Reprod Sci Hematological and Neurological Expressed 1 Promotes Tumor Progression Through mTOR Signaling in Ovarian Cancer Authors - Pin Li KD Validated View Article

HN1 Polyclonal antibody

HN1 Polyclonal Antibody for IHC, IP, WB,ELISA

Validation Data Gallery

WB analysis of HeLa using 14914-1-AP

WB analysis of human testis using 14914-1-AP

WB analysis of HepG2 using 14914-1-AP

WB analysis of A431 using 14914-1-AP

WB analysis of human brain using 14914-1-AP

WB analysis of mouse testis using 14914-1-AP

WB analysis of mouse colon using 14914-1-AP

WB analysis of LNCaP using 14914-1-AP

IP experiment of MCF-7 using 14914-1-AP

IHC staining of human ovary tumor using 14914-1-AP

IHC staining of human ovary tumor using 14914-1-AP

IHC staining of human breast cancer using 14914-1-AP

IHC staining of human breast cancer using 14914-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

HN1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by interacting with STMN1.

Increased expression of hematological and neurological expressed 1 (HN1) is associated with a poor prognosis of hepatocellular carcinoma and its knockdown inhibits cell growth and migration partly by down-regulation of c-Met.

Matrine exerts an anti-tumor effect via regulating HN1 in triple breast cancer both in vitro and in vivo

Epigenetic inhibition of CTCF by HN1 promotes dedifferentiation and stemness of anaplastic thyroid cancer

Hematological and Neurological Expressed 1 Promotes Tumor Progression Through mTOR Signaling in Ovarian Cancer