HNRNPA0 Polyclonal antibody

HNRNPA0 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 10848-1-AP

Synonyms

hnRNP A0, HNRNPA0, HNRPA0



Tested Applications

Positive WB detected inHeLa cells, fetal human brain tissue, mouse brain tissue, Jurkat cells, NIH/3T3 cells
Positive IP detected inmouse brain tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10848-1-AP targets HNRNPA0 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPA0 fusion protein Ag1312
Full Name heterogeneous nuclear ribonucleoprotein A0
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC019271
Gene Symbol HNRNPA0
Gene ID (NCBI) 10949
RRIDAB_2117066
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRNPA0 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Protocols

Product Specific Protocols
WB protocol for HNRNPA0 antibody 10848-1-APDownload protocol
IF protocol for HNRNPA0 antibody 10848-1-APDownload protocol
IP protocol for HNRNPA0 antibody 10848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xiaochen (Verified Customer) (07-31-2023)

hnrnpa0 has many subtypes and thus got two bands, I think it is acceptable. Antibody quality is prety good.

  • Applications: Western Blot
HNRNPA0 Antibody Western Blot validation ( dilution) in (Cat no:10848-1-AP)