HNRNPA3 Polyclonal antibody
HNRNPA3 Polyclonal Antibody for WB, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25142-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, Jurkat cells |
| Positive IF detected in | mouse colon tissue, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
25142-1-AP targets HNRNPA3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, monkey, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HNRNPA3 fusion protein Ag18248 |
| Full Name | heterogeneous nuclear ribonucleoprotein A3 |
| Calculated Molecular Weight | 378 aa, 40 kDa |
| Observed Molecular Weight | 37-40 kDa |
| GenBank Accession Number | BC113470 |
| Gene Symbol | HNRNPA3 |
| Gene ID (NCBI) | 220988 |
| RRID | AB_2879921 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HNRNPA3 also named as hnRNP A3 or heterogeneous nuclear ribonucleoprotein A3 is a 378 amino acid protein, which contains 2 RRM domains and localizes in nucleus. HNRNPA3 is a component of the spliceosome C complex and play a role in cytoplasmic trafficking of RNA. Binds to the cis-acting response element.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HNRNPA3 antibody 25142-1-AP | Download protocol |
| IF protocol for HNRNPA3 antibody 25142-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism. | ||
Arterioscler Thromb Vasc Biol Novel Long Noncoding RNA, Macrophage Inflammation-Suppressing Transcript (MIST), Regulates Macrophage Activation During Obesity. | ||
Neurobiol Dis ALS-linked cytoplasmic FUS assemblies are compositionally different from physiological stress granules and sequester hnRNPA3, a novel modifier of FUS toxicity. | ||
Sci China Life Sci Identification of mecciRNAs and their roles in the mitochondrial entry of proteins. | ||
Mediators Inflamm Integrated Functional Analysis of the Nuclear Proteome of Classically and Alternatively Activated Macrophages. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tatyana (Verified Customer) (01-21-2023) | Works well in ICC and WB in human cells. Also gives a weak but specific signal in the mouse CNS tissue (spinal cord). WB: 4% milk in TBST and semi-dry transfer; detects a double band at ~55kDa ICC: 5% goat serum in PBST; mainly nuclear IHC: 4% PFA fixed and paraffinised sections; standard antigen retrieval and DAB detection; mainly nuclear Used at 1:1000, with incubation overnight at 4C, in all applications
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