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  • Featured Product
  • KD/KO Validated

HNRNPU Polyclonal antibody

HNRNPU Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16365-1-AP

Synonyms

hnRNP U, HNRNPU, HNRPU, p120, pp120, SAF A, SAFA, Scaffold attachment factor A, U21.1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using HNRNPU Polyclonal antibody (16365-1-AP)

    WB analysis of HEK-293 using 16365-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16365-1-AP (HNRNPU antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HNRNPU Polyclonal antibody (16365-1-AP)

    WB analysis of HeLa using 16365-1-AP

    WB result of HNRNPU antibody (16365-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPU transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using HNRNPU Polyclonal antibody (16365-1-AP)

    WB analysis of HeLa using 16365-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16365-1-AP (HNRNPU antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HNRNPU Polyclonal antibody (16365-1-AP)

    WB analysis using 16365-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16365-1-AP (HNRNPU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using HNRNPU Polyclonal antibody (16365-1-AP)

    IP experiment of HeLa using 16365-1-AP

    IP result of anti-HNRNPU (IP:16365-1-AP, 4ug; Detection:16365-1-AP 1:8000) with HeLa cells lysate 2000 ug.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using HNRNPU Polyclonal antibody (16365-1-AP)

    IHC staining of mouse kidney using 16365-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16365-1-AP (HNRNPU antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using HNRNPU Polyclonal antibody (16365-1-AP)

    IHC staining of mouse kidney using 16365-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16365-1-AP (HNRNPU antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPU Polyclonal antibody (16365-1-AP)

    IF Staining of HeLa using 16365-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16365-1-AP (HNRNPU antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using HNRNPU Polyclonal antibody (16365-1-AP)

    IF Staining of mouse testis using 16365-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 16365-1-AP (HNRNPU antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using HNRNPU Polyclonal antibody (16365-1-AP)

    FC experiment of HepG2 using 16365-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug HNRNPU antibody (16365-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, mouse testis tissue
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16365-1-AP targets HNRNPU in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPU fusion protein Ag9151
Full Name heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC003367
Gene Symbol HNRNPU
Gene ID (NCBI) 3192
RRIDAB_10596481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).

Protocols

Product Specific Protocols
WB protocol for HNRNPU antibody 16365-1-APDownload protocol
IHC protocol for HNRNPU antibody 16365-1-APDownload protocol
IF protocol for HNRNPU antibody 16365-1-APDownload protocol
IP protocol for HNRNPU antibody 16365-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dev Cell

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

Authors - Yanni Ma
View Article
humanIF

Cancer Res

DIS3L2 promotes progression of hepatocellular carcinoma via hnRNP U-mediated alternative splicing.

Authors - Songge Xing
View Article
humanIP,IF

mSystems

Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia.

Authors - Xiaoqing Liu
View Article

Cell Prolif

Protein phosphatase 4 plays dual roles during cell proliferation.

Authors - Xiuqing Huang
View Article
humanWB

J Exp Clin Cancer Res

A novel protein encoded by circHNRNPU promotes multiple myeloma progression by regulating the bone marrow microenvironment and alternative splicing.

Authors - Xiaozhu Tang
  • KD Validated
View Article
humanIHC,WB

Front Oncol

The hnRNPK/A1/R/U Complex Regulates Gene Transcription and Translation and is a Favorable Prognostic Biomarker for Human Colorectal Adenocarcinoma.

Authors - Yixin Li
View Article