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CoraLite® Plus 488-conjugated HO-1/HMOX1 Monoclonal antibody

HO-1/HMOX1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D10A5

Cat no : CL488-66743

Synonyms

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HO-1/HMOX1, HSP32

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using CoraLite® Plus 488-conjugated HO-1/HMOX1 Monoclona (CL488-66743)

    IF Staining of human liver using CL488-66743

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CL488-66743 (HO-1/HMOX1 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using CoraLite® Plus 488-conjugated HO-1/HMOX1 Monoclona (CL488-66743)

    IF Staining of human liver using CL488-66743

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CL488-66743 (HO-1/HMOX1 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CoraLite® Plus 488-conjugated HO-1/HMOX1 Monoclona (CL488-66743)

    IF Staining of mouse spleen using CL488-66743

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 HO-1/HMOX1 antibody (CL488-66743, Clone: 2D10A5 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated HO-1/HMOX1 Monoclona (CL488-66743)

    FC experiment of HeLa using CL488-66743

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HO-1/HMOX1 (CL488-66743, Clone:2D10A5) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inhuman liver tissue, mouse spleen tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66743 targets HO-1/HMOX1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HO-1/HMOX1 fusion protein Ag21296
Full Name heme oxygenase (decycling) 1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC001491
Gene Symbol HMOX1
Gene ID (NCBI) 3162
RRIDAB_2883379
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HO-1/HMOX1 antibody CL488-66743Download protocol
Standard Protocols
Click here to view our Standard Protocols