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HOOK2 Polyclonal antibody

HOOK2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12458-1-AP

Synonyms

h hook2, hHK2, HK2, hook homolog 2 (Drosophila), HOOK2, Protein Hook homolog 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HOOK2 Polyclonal antibody (12458-1-AP)

    WB analysis using 12458-1-AP

    SH-SY5Y cell lysates were subjected to SDS PAGE followed by western blot with 12458-1-AP (HOOK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using HOOK2 Polyclonal antibody (12458-1-AP)

    IHC staining of mouse colon using 12458-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12458-1-AP (HOOK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12458-1-AP targets HOOK2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOOK2 fusion protein Ag3124
Full Name hook homolog 2 (Drosophila)
Calculated Molecular Weight 717 aa, 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC012443
Gene Symbol HOOK2
Gene ID (NCBI) 29911
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HOOK2 antibody 12458-1-APDownload protocol
IHC protocol for HOOK2 antibody 12458-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols