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  • Featured Product
  • KD/KO Validated

HPD Polyclonal antibody

HPD Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17004-1-AP

Synonyms

4 HPPD, 4HPPD, GLOD3, HPD, HPPDase, PPD

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using HPD Polyclonal antibody (17004-1-AP)

    WB analysis of HepG2 using 17004-1-AP

    WB result of HPD antibody (17004-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HPD transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using HPD Polyclonal antibody (17004-1-AP)

    WB analysis using 17004-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17004-1-AP (HPD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HPD Polyclonal antibody (17004-1-AP)

    WB analysis using 17004-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17004-1-AP (HPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using HPD Polyclonal antibody (17004-1-AP)

    WB analysis of HepG2 using 17004-1-AP

    WB result of HPD antibody (17004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HPD transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human liver tissue using HPD Polyclonal antibody (17004-1-AP)

    IHC staining of human liver using 17004-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17004-1-AP (HPD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using HPD Polyclonal antibody (17004-1-AP)

    IHC staining of human liver using 17004-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17004-1-AP (HPD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HPD Polyclonal antibody (17004-1-AP)

    IF Staining of HepG2 using 17004-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 17004-1-AP (HPD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17004-1-AP targets HPD in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HPD fusion protein Ag10700
Full Name 4-hydroxyphenylpyruvate dioxygenase
Calculated Molecular Weight 393 aa, 45 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC024287
Gene Symbol HPD
Gene ID (NCBI) 3242
RRIDAB_2264310
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HPD antibody 17004-1-APDownload protocol
IHC protocol for HPD antibody 17004-1-APDownload protocol
IF protocol for HPD antibody 17004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nephrol Dial Transplant

Key enzyme in charge of ketone reabsorption of renal tubular SMCT1 may be a new target in diabetic kidney disease

Authors - Zhenhong Guo
View Article