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HS2ST1 Polyclonal antibody

HS2ST1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12453-1-AP

Synonyms

2 O sulfotransferase, 2OST, HS2ST, HS2ST1, KIAA0448

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using HS2ST1 Polyclonal antibody (12453-1-AP)

    WB analysis of human liver using 12453-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12453-1-AP (HS2ST1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using HS2ST1 Polyclonal antibody (12453-1-AP)

    WB analysis of human heart using 12453-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12453-1-AP (HS2ST1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using HS2ST1 Polyclonal antibody (12453-1-AP)

    WB analysis of human brain using 12453-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12453-1-AP (HS2ST1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using HS2ST1 Polyclonal antibody (12453-1-AP)

    WB analysis of PC-3 using 12453-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12453-1-AP (HS2ST1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using HS2ST1 Polyclonal antibody (12453-1-AP)

    IHC staining of human lymphoma using 12453-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 12453-1-AP (HS2ST1 antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inhuman liver tissue, human brain tissue, human heart tissue, PC-3 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

12453-1-AP targets HS2ST1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HS2ST1 fusion protein Ag3111
Full Name heparan sulfate 2-O-sulfotransferase 1
Calculated Molecular Weight 229 aa, 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC025990
Gene Symbol HS2ST1
Gene ID (NCBI) 9653
RRIDAB_2119312
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HS2ST1 antibody 12453-1-APDownload protocol
IHC protocol for HS2ST1 antibody 12453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Medicine (Baltimore)

Two novel predictive biomarkers for osteosarcoma and glycolysis pathways: A profiling study on HS2ST1 and SDC3

Authors - Guozhi Yang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Bettina (Verified Customer) (08-05-2022)

Weak detection

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human pancreatic cancer cell line
HS2ST1 Antibody Western Blot validation (1:1000 dilution) in Human pancreatic cancer cell line (Cat no:12453-1-AP)