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HSD11B1 Polyclonal antibody

HSD11B1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10928-1-AP

Synonyms

11 beta HSD1, 11 DH, HDL, HSD11, HSD11B, HSD11B1, HSD11L, SDR26C1



Tested Applications

Positive WB detected inL02 cells, mouse liver tissue, Jurkat cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10928-1-AP targets HSD11B1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSD11B1 fusion protein Ag1361
Full Name hydroxysteroid (11-beta) dehydrogenase 1
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC012593
Gene Symbol HSD11B1
Gene ID (NCBI) 3290
RRIDAB_2119527
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSD11B1(Corticosteroid 11-beta-dehydrogenase isozyme 1) is also named as HSD11, HSD11L,11-DH and 11-beta-HSD1.It is a microsomal enzyme, which acts mainly as a NADP(H)- dependent reductase converting inactive cortisone (E) to active cortisol (F), thus regulating intracellular F access to glucocorticoid receptor. This enzyme is widely expressed not only in liver and adipose tissue, but also in adrenal, ovary and decidua.Western blot analysis in human liver, using a specific11-beta-HSD1 antibody, showed two bands at approximately 34 and 68 kDa confirming the dimeric nature of 11-beta-HSD1 in liver. In omental adipose tissue, an additional band of 50 kDa was strongly expressed(PMID:16914598).

Protocols

Product Specific Protocols
WB protocol for HSD11B1 antibody 10928-1-APDownload protocol
IF protocol for HSD11B1 antibody 10928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Brain Behav Immun

HMGB1-mediated differential response on hippocampal neurotransmitter disorder and neuroinflammation in adolescent male and female mice following cold exposure.

Authors - Bin Xu
humanWB

J Assist Reprod Genet

Effect of luteinizing hormone concentration on transcriptome and subcellular organelle phenotype of ovarian granulosa cells.

Authors - Yu-Ting Wan
mouseIF,WB,IHC

Int J Biol Sci

11β-Hydroxysteroid Dehydrogenase Type 1 Facilitates Osteoporosis by Turning on Osteoclastogenesis through Hippo Signaling

Authors - Hanwen Li
humanWB

Gynecol Endocrinol

N6-methyladenosine demethylase FTO related to hyperandrogenism in PCOS via AKT pathway

Authors - Yuan-Xue Jing