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HSDL1 Polyclonal antibody

HSDL1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC

Conjugate

Unconjugated

Cat no : 16988-1-AP

Synonyms

HSDL1, SDR12C3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HSDL1 Polyclonal antibody (16988-1-AP)

    WB analysis using 16988-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16988-1-AP (HSDL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HSDL1 Polyclonal antibody (16988-1-AP)

    WB analysis of HEK-293 using 16988-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16988-1-AP (HSDL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using HSDL1 Polyclonal antibody (16988-1-AP)

    WB analysis of fetal human brain using 16988-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 16988-1-AP (HSDL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HSDL1 Polyclonal antibody (16988-1-AP)

    IHC staining of human breast cancer using 16988-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16988-1-AP (HSDL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HSDL1 Polyclonal antibody (16988-1-AP)

    IHC staining of human breast cancer using 16988-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16988-1-AP (HSDL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HSDL1 Polyclonal antibody (16988-1-AP)

    IHC staining of human kidney using 16988-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16988-1-AP (HSDL1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HSDL1 Polyclonal antibody (16988-1-AP)

    IHC staining of human kidney using 16988-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16988-1-AP (HSDL1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using HSDL1 Polyclonal antibody (16988-1-AP)

    IHC staining of human lung using 16988-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16988-1-AP (HSDL1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using HSDL1 Polyclonal antibody (16988-1-AP)

    IHC staining of human lung using 16988-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16988-1-AP (HSDL1 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, fetal human brain tissue
Positive IHC detected inhuman breast cancer tissue, human kidney tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16988-1-AP targets HSDL1 in WB, IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSDL1 fusion protein Ag10588
Full Name hydroxysteroid dehydrogenase like 1
Calculated Molecular Weight 330 aa, 37 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC018084
Gene Symbol HSDL1
Gene ID (NCBI) 83693
RRIDAB_2120235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HSDL1 antibody 16988-1-APDownload protocol
IHC protocol for HSDL1 antibody 16988-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Sci Adv

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Authors - Odetta Antico
View Article