HSPB7 Polyclonal antibody

HSPB7 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15700-1-AP

Synonyms

cvHSP, Heat shock protein beta 7, HSPB7



Tested Applications

Positive WB detected inHepG2 cells, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15700-1-AP targets HSPB7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSPB7 fusion protein Ag8328
Full Name heat shock 27kDa protein family, member 7 (cardiovascular)
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 18-23 kDa
GenBank Accession NumberBC006319
Gene Symbol HSPB7
Gene ID (NCBI) 27129
RRIDAB_2233322
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HSPB7 antibody 15700-1-APDownload protocol
IHC protocol for HSPB7 antibody 15700-1-APDownload protocol
IP protocol for HSPB7 antibody 15700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Acta Neuropathol Commun

Homozygous expression of the myofibrillar myopathy-associated p.W2710X filamin C variant reveals major pathomechanisms of sarcomeric lesion formation.

Authors - Julia Schuld
mouseWB

Exp Cell Res

Isoform-specific functions of synaptopodin-2 variants in cytoskeleton stabilization and autophagy regulation in muscle under mechanical stress.

Authors - Keerthika Lohanadan
humanWB,IHC,IF

Int J Oncol

Downregulation of the tumor suppressor HSPB7, involved in the p53 pathway, in renal cell carcinoma by hypermethylation.

Authors - Jiaying Lin