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Hemopexin Monoclonal antibody

Hemopexin Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, pig and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3A9D6

Cat no : 66479-1-Ig

Synonyms

Beta 1B glycoprotein, hemopexin, HPX

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Hemopexin Monoclonal antibody (66479-1-Ig)

    WB analysis of human plasma using 66479-1-Ig

    0.7 μL human plasma was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig plasma using Hemopexin Monoclonal antibody (66479-1-Ig)

    WB analysis of pig plasma using 66479-1-Ig

    0.7 μL pig plasma was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat serum using Hemopexin Monoclonal antibody (66479-1-Ig)

    WB analysis of rat serum using 66479-1-Ig

    0.7 μL rat serum was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Hemopexin Monoclonal antibody (66479-1-Ig)

    WB analysis of human placenta using 66479-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Hemopexin Monoclonal antibody (66479-1-Ig)

    IHC staining of human liver using 66479-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Hemopexin Monoclonal antibody (66479-1-Ig)

    IHC staining of human liver using 66479-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Hemopexin Monoclonal antibody (66479-1-Ig)

    IHC staining of human placenta using 66479-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Hemopexin Monoclonal antibody (66479-1-Ig)

    IF Staining of human liver cancer using 66479-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Hemopexin antibody (66479-1-Ig, Clone: 3A9D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using Hemopexin Monoclonal antibody (66479-1-Ig)

    FC experiment of HepG2 using 66479-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human Hemopexin (66479-1-Ig, Clone:3A9D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inhuman plasma tissue, human placenta tissue, rat serum tissue, pig plasma tissue
Positive IHC detected inhuman liver tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

66479-1-Ig targets Hemopexin in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, rat, pig samples.

Tested Reactivity Human, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Hemopexin fusion protein Ag8533
Full Name hemopexin
Calculated Molecular Weight 254 aa, 29 kDa
Observed Molecular Weight 65-75 kDa
GenBank Accession NumberBC005395
Gene Symbol HPX
Gene ID (NCBI) 3263
RRIDAB_2881845
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.

Protocols

Product Specific Protocols
WB protocol for Hemopexin antibody 66479-1-IgDownload protocol
IHC protocol for Hemopexin antibody 66479-1-IgDownload protocol
IF protocol for Hemopexin antibody 66479-1-IgDownload protocol
FC protocol for Hemopexin antibody 66479-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Matrix Biol

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Authors - Emna Ouni
View Article