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  • KD/KO Validated

HuR/ELAVL1 Polyclonal antibody

HuR/ELAVL1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, CLIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 11910-1-AP

Synonyms

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG



Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11910-1-AP targets HuR/ELAVL1 in WB, RIP, IP, IHC, IF, FC, CoIP, CLIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HuR/ELAVL1 fusion protein Ag2249
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Calculated Molecular Weight 326 aa, 36 kDa
Observed Molecular Weight 30-37 kDa
GenBank Accession NumberBC003376
Gene Symbol ELAVL1
Gene ID (NCBI) 1994
RRIDAB_11182183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

Protocols

Product Specific Protocols
WB protocol for HuR/ELAVL1 antibody 11910-1-APDownload protocol
IHC protocol for HuR/ELAVL1 antibody 11910-1-APDownload protocol
IF protocol for HuR/ELAVL1 antibody 11910-1-APDownload protocol
IP protocol for HuR/ELAVL1 antibody 11910-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cancer

SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers

Authors - Lei Shi
humanWB

Nat Protoc

Global analysis of RNA-binding protein dynamics by comparative and enhanced RNA interactome capture.

Authors - Joel I Perez-Perri
humanIF

Acta Neuropathol

Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis.

Authors - Sandra Diaz-Garcia
mouseWB

Mol Cell

Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.

Authors - Jing Wen
humanWB

Autophagy

m6A reader YTHDC1 modulates autophagy by targeting SQSTM1 in diabetic skin

Authors - Diefei Liang
  • KD Validated
humanWB

Nat Commun

Discovery of RNA-binding proteins and characterization of their dynamic responses by enhanced RNA interactome capture.

Authors - Joel I Perez-Perri

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Adrianna (Verified Customer) (10-24-2022)

This antibody works very well - strong detection without unspecific bands. I usually incubate it over night at 4 deg C.

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MCF7
FH

PK (Verified Customer) (07-08-2022)

Very Good

  • Applications: Western Blot
  • Cell Tissue Type: H9C2
HuR/ELAVL1 Antibody Western Blot validation ( dilution) in H9C2 (Cat no:11910-1-AP)
FH

S (Verified Customer) (04-19-2021)

Excellent!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: AC16
HuR/ELAVL1 Antibody Western Blot validation (1:1000 dilution) in AC16 (Cat no:11910-1-AP)
FH

S (Verified Customer) (05-18-2020)

Good Antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: AC16
HuR/ELAVL1 Antibody Western Blot validation (1:1000 dilution) in AC16 (Cat no:11910-1-AP)
FH

CHAO (Verified Customer) (01-30-2020)

Weak band for the heart lysates

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart
FH

Gaston (Verified Customer) (05-16-2019)

Antibody worked very well for wester blot detection and immunofluorescence in Huh7 and SH-SY5Y cells. Did not observe any signs of deteriorating detection when stored at -20 for over two years.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:250; 1:6500
  • Cell Tissue Type: Huh7, SH-SY5Y, Vero
HuR/ELAVL1 Antibody Western Blot,Immunofluorescence, validation (1:250; 1:6500 dilution) in Huh7, SH-SY5Y, Vero (Cat no:11910-1-AP)