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  • KD/KO Validated

HuR/ELAVL1 Monoclonal antibody

HuR/ELAVL1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A11H2

Cat no : 66549-1-Ig

Synonyms

ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, A549 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, NCI-H1299 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66549-1-Ig targets HuR/ELAVL1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HuR/ELAVL1 fusion protein Ag2249
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Calculated Molecular Weight 326 aa, 36 kDa
Observed Molecular Weight 34-37 kDa
GenBank Accession NumberBC003376
Gene Symbol ELAVL1
Gene ID (NCBI) 1994
RRIDAB_2881911
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

Protocols

Product Specific Protocols
WB protocol for HuR/ELAVL1 antibody 66549-1-IgDownload protocol
IHC protocol for HuR/ELAVL1 antibody 66549-1-IgDownload protocol
IF protocol for HuR/ELAVL1 antibody 66549-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta Mol Cell Res

IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5

Authors - Junfeng Du
mouseWB,IF

Mol Cell Endocrinol

ELAV-like RNA binding protein 1 regulates osteogenesis in diabetic osteoporosis: Involvement of divalent metal transporter 1.

Authors - Yuanfei Ren
humanWB

Cell Signal

Butyrate mediated regulation of RNA binding proteins in the post-transcriptional regulation of inflammatory gene expression.

Authors - Aydan Torun
humanWB

J Int Med Res

Long non-coding RNA DEPDC1-AS1 promotes proliferation and migration of human gastric cancer cells HGC-27 via the human antigen R-F11R pathway.

Authors - Wei Xu
humanWB

Cell Death Dis

LncGMDS-AS1 promotes the tumorigenesis of colorectal cancer through HuR-STAT3/Wnt axis

Authors - Deji Ye
  • KD Validated
humanIF

Oncol Lett

Stress granules affect the sensitivity of renal cancer cells to sorafenib by sequestering and stabilizing COX‑2 mRNA

Authors - Huiqi Dai