Human CD23/FCER2 ELISA Kit

Sensitivity

2.2 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Human

Cat no : KE00107

Synonyms

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor



Product Information

KE00107 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD23 ELISA kit is to be used to detect and quantify protein levels of endogenous CD23. The assay recognizes human CD23. An antibody specific for CD23 has been pre-coated onto the microwells. The CD23 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for CD23 is added to detect the captured CD23 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human CD23/FCER2 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Urine
Assay type Sandwich
Sensitivity 2.2 pg/mL
Range 31.25-2000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 2208

Recovery

Sample TypeAverageRange
Urine106%96%-122%
Human plasma101%87%-119%
Cell culture supernatants104%82%-117%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120879.941.24.7
220216.99.94.6
32049.94.79.5

InterAssay

Samplenmean ( pg/mL)SDCV%
124849.472.48.5
224209.618.58.8
32448.24.18.6

Background Information

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells. CD23 can be cleaved into soluble fragment (soluble CD23, sCD23) of various sizes displaying pleiotropic biological activities. Increased plasma levels of sCD23 have been reported in allergic disorders, acute viral diseases, lepromatous leprosy, B-CLL, and inflammatory diseases like RA, SLE and sarcoidosis.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor