Human IL-31 ELISA Kit

Sensitivity

7.4 pg/mL

Range

31.25 - 2000 pg/mL

Reactivity

Human

Cat no : KE00086

Synonyms

IL 31, IL31, IL-31, interleukin 31



Product Information

KE00086 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human IL31 ELISA kit is to be used to detect and quantify protein levels of endogenous human IL31. The assay recognizes human IL31. An antibody specific for human IL31 has been pre-coated onto the microwells. The human IL31 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human IL31 is added to detect the captured human IL31 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human IL-31 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 7.4 pg/mL
Range 31.25 - 2000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 386653

Recovery

Sample TypeAverageRange
Human serum91%74%-117%
Cell culture supernatants91%83%-113%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12041.01.74.2
220431.610.72.5
3201,731.552.73.0

InterAssay

Samplenmean ( pg/mL)SDCV%
12437.43.38.9
224415.915.13.6
3241,726.274.84.3

Background Information

Interleukin-31 (IL-31) is a newly discovered four-helix bundle cytokine and expressed by several types of cells under both normal and disease state, such as activated CD4+T cells, mast cells, monocytes, macrophages, immature and mature monocyte-derived dendritic cells and so on. IL-31 signals through a heterodimeric receptor complex consisting of the IL31 receptor alpha (IL31Rα) and the oncostatin M receptor (OSMR). IL-31 plays a predominant function in the mediation of inflammatory and lymphoma-associated itch. Increased IL-31 expression has been detected in inflamed tissues from patients with atopic dermatitis, bowel diseases, allergic asthma and rhinitis.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms IL 31, IL31, IL-31, interleukin 31