Human LBP ELISA Kit

Sensitivity

0.02 ng/mL

Range

3.13-200 ng/mL

Reactivity

Human

Cat no : KE00134

Synonyms

LBP



Product Information

KE00134 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human LBP ELISA kit is to be used to detect and quantify protein levels of endogenous human LBP. The assay recognizes human LBP. An antibody specific for human LBP has been pre-coated onto the microwells. The human LBP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human LBP is added to detect the captured human LBP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .

Product name Human LBP ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.02 ng/mL
Range 3.13-200 ng/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3929

Recovery

Sample TypeAverageRange
Human serum95%84% -112%
Cell culture supernatants102%84% -119%

IntraAssay

Samplenmean ( ng/mL)SDCV%
12012.20.86.4
22048.42.85.7
32089.83.13.5

InterAssay

Samplenmean ( ng/mL)SDCV%
12411.60.65.4
22444.82.04.4
32487.65.15.9

Background Information

LBP (lipopolysaccharide-binding protein) is a serum glycoprotein belonging to the BPI/LBP/Plunc superfamily and BPI/LBP family. It is an acute-phase protein, predominantly synthesized by hepatocytes. LBP is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS) which is a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms LBP

Publications

SpeciesSample TypeTitle
human

J Exp Clin Cancer Res

Gastric cancer-derived LBP promotes liver metastasis by driving intrahepatic fibrotic pre-metastatic niche formation

Authors - Li Xie