Human/Mouse/Rat PIP5K1A ELISA Kit
Sensitivity
0.008 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human/Mouse/Rat
Cat no : KE00227
Synonyms
PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha
Validation Data Gallery
Product Information
KE00227 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PIP5K1A ELISA kit is to be used to detect and quantify protein levels of endogenous PIP5K1A. The assay recognizes human/mouse/rat PIP5K1A. An antibody specific for PIP5K1A has been pre-coated onto the microwells. The PIP5K1A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for PIP5K1A is added to detect the captured PIP5K1A protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse/Rat PIP5K1A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Mouse serum, Rat serum and Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.008 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8394 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 87% | 80%-98% |
| Mouse serum | 102% | 76%-120% |
| Rat serum | 86% | 78%-97% |
| Cell lysate | 86% | 75%-99% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 0.30 | 0.02 | 8.4 |
| 2 | 20 | 1.17 | 0.03 | 2.6 |
| 3 | 20 | 4.92 | 0.14 | 2.9 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 0.28 | 0.02 | 6.5 |
| 2 | 24 | 1.20 | 0.08 | 7.0 |
| 3 | 24 | 5.57 | 0.38 | 6.8 |
Background Information
Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha |