Human TIMP2 ELISA Kit

Sensitivity

0.3 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Human

Cat no : KE00162

Synonyms

CSC 21K, Metalloproteinase inhibitor 2, TIMP 2, TIMP2



Product Information

KE00162 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TIMP2 ELISA kit is to be used to detect and quantify protein levels of endogenous TIMP2. The assay recognizes human TIMP2. An antibody specific for TIMP2 has been pre-coated onto the microwells. The TIMP2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human TIMP2 is added to detect the captured human TIMP2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human TIMP2 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Urine, Saliva
Assay type Sandwich
Sensitivity 0.3 pg/mL
Range 31.25-2000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 7077

Recovery

Sample TypeAverageRange
Human serum99%72%-119%
Cell culture supernatants97%90%-116%
Urine107%98%-117%
Saliva103%94%-113%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120987.540.14.1
220255.87.93.1
32084.93.23.8

InterAssay

Samplenmean ( pg/mL)SDCV%
124971.031.53.2
224262.87.52.8
32488.72.42.7

Background Information

TIMP2, a 21-kDa protein, is a member of the family of matrix metalloproteinase inhibitors, which contains four members (TIMP1, TIMP2, TIMP3, and TIMP4). Tissue inhibitors of metalloproteinases (TIMPs) are multifaceted molecules that exhibit properties beyond their classical proteinase inhibitory function. TIMP-2 is the only TIMP functioning as both an MMP inhibitor and an activator, because it can interact with membrane type 1 MMP and pro-MMP2 to facilitate the activation of pro-MMP2 to its active form. TIMP2 can regulate many physiological progression such as tumor growth, metastasis, and angiogenesis through matrix metalloproteinases (MMPs). TIMP2 knockout resulted in constitutive MMP9 activation abundantly and adverse MI remodeling in vivo.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms CSC 21K, Metalloproteinase inhibitor 2, TIMP 2, TIMP2

Publications

SpeciesSample TypeTitle
humanserum, urine

J Crit Care

A translational study of Galectin-3 as an early biomarker and potential therapeutic target for ischemic-reperfusion induced acute kidney injury.

Authors - Haibing Sun