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IFIT1L Polyclonal antibody

IFIT1L Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21483-1-AP

Synonyms

IFIT1B, IFIT1L

Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using IFIT1L Polyclonal antibody (21483-1-AP)

    WB analysis of RAW 264.7 using 21483-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 21483-1-AP (IFIT1L Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IFIT1L Polyclonal antibody (21483-1-AP)

    IHC staining of mouse testis using 21483-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21483-1-AP (IFIT1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IFIT1L Polyclonal antibody (21483-1-AP)

    IHC staining of mouse testis using 21483-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21483-1-AP (IFIT1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using IFIT1L Polyclonal antibody (21483-1-AP)

    IHC staining of human testis using 21483-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21483-1-AP (IFIT1L Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using IFIT1L Polyclonal antibody (21483-1-AP)

    IF Staining of HEK-293 using 21483-1-AP

    Immunofluorescent analysis of HEK-293 cells using 21483-1-AP (IFIT1L antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using IFIT1L Polyclonal antibody (21483-1-AP)

    IF Staining of RAW 264.7 using 21483-1-AP

    Immunofluorescent analysis of RAW 264.7 cells using 21483-1-AP (IFIT1L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inRAW 264.7 cells
Positive IHC detected inmouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21483-1-AP targets IFIT1L in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFIT1L fusion protein Ag15765
Full Name interferon-induced protein with tetratricopeptide repeats 1-like
Calculated Molecular Weight 474 aa, 55 kDa
Observed Molecular Weight 55-65 kDa
GenBank Accession NumberBC150189
Gene Symbol IFIT1L
Gene ID (NCBI) 439996
RRIDAB_2878867
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IFIT1L antibody 21483-1-APDownload protocol
IHC protocol for IFIT1L antibody 21483-1-APDownload protocol
IF protocol for IFIT1L antibody 21483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols