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IFNGR1 Monoclonal antibody

IFNGR1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1H3A4

Cat no : 66704-1-Ig

Synonyms

CD119, CDw119, FLJ45734, IFN gamma R1, IFN gamma receptor 1, IFNGR, IFNGR1, interferon gamma receptor 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IFNGR1 Monoclonal antibody (66704-1-Ig)

    WB analysis of HeLa using 66704-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using IFNGR1 Monoclonal antibody (66704-1-Ig)

    WB analysis of HEK-293 using 66704-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using IFNGR1 Monoclonal antibody (66704-1-Ig)

    WB analysis of T-47D using 66704-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using IFNGR1 Monoclonal antibody (66704-1-Ig)

    WB analysis of Jurkat using 66704-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IFNGR1 Monoclonal antibody (66704-1-Ig)

    IHC staining of human tonsillitis using 66704-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66704-1-Ig (IFNGR1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, T-47D cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66704-1-Ig targets IFNGR1 in WB,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IFNGR1 fusion protein Ag27085
Full Name interferon gamma receptor 1
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC005333
Gene Symbol IFNGR1
Gene ID (NCBI) 3459
RRIDAB_2882056
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)

Protocols

Product Specific Protocols
WB protocol for IFNGR1 antibody 66704-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols