IGFBP5 Polyclonal antibody

IGFBP5 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55205-1-AP

Synonyms

IBP 5, IBP5, IGF binding protein 5, IGFBP 5, IGFBP5



Tested Applications

Positive WB detected inSKOV-3 cells, mouse embryo tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55205-1-AP targets IGFBP5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name insulin-like growth factor binding protein 5
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberNM_000599
Gene Symbol IGFBP5
Gene ID (NCBI) 3488
RRIDAB_2736835
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5.

Protocols

Product Specific Protocols
WB protocol for IGFBP5 antibody 55205-1-APDownload protocol
IHC protocol for IGFBP5 antibody 55205-1-APDownload protocol
IF protocol for IGFBP5 antibody 55205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Theranostics

Material-induced Senescence (MIS): Fluidity Induces Senescent Type Cell Death of Lung Cancer Cells via Insulin-Like Growth Factor Binding Protein 5.

Authors - Sharmy Saimon Mano
humanWB

Oncogene

Fibulin-3 promotes muscle-invasive bladder cancer.

Authors - A L Han
humanIF

Breast Cancer Res

Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors

Authors - Wei Yang
mouseWB

Transl Res

PMID: 30392876

Authors - Lin Xihua
humanWB

J Proteomics

Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.

Authors - Sun-Xia Chen
ratWB

Am J Physiol Heart Circ Physiol

Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5.

Authors - Guangrui Lai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Margarita (Verified Customer) (06-06-2023)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: CWR22Rv1 cells
IGFBP5 Antibody Immunofluorescence validation (1:100 dilution) in CWR22Rv1 cells (Cat no:55205-1-AP)