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IL-12RB1 Monoclonal antibody

IL-12RB1 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1E10E10

Cat no : 66829-1-Ig

Synonyms

CD212, IL 12 receptor beta component, IL 12 receptor subunit beta 1, IL 12R beta 1, IL 12R BETA1, IL 12R subunit beta 1, IL 12RB1, IL12R, IL12RB, IL12RB1, IL-12RB1

Validation Data Gallery

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  • Western Blot (WB) analysis of Ramos cells using IL-12RB1 Monoclonal antibody (66829-1-Ig)

    WB analysis of Ramos using 66829-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IL-12RB1 Monoclonal antibody (66829-1-Ig)

    WB analysis using 66829-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using IL-12RB1 Monoclonal antibody (66829-1-Ig)

    WB analysis of Jurkat using 66829-1-Ig

    WB result of IL-12RB1 antibody (66829-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-12RB1 transfected Jurkat cells.

  • Western Blot (WB) analysis of Raji cells using IL-12RB1 Monoclonal antibody (66829-1-Ig)

    WB analysis of Raji using 66829-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using IL-12RB1 Monoclonal antibody (66829-1-Ig)

    WB analysis of THP-1 using 66829-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using IL-12RB1 Monoclonal antibody (66829-1-Ig)

    WB analysis of Jurkat using 66829-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of K-562 cells using IL-12RB1 Monoclonal antibody (66829-1-Ig)

    FC experiment of K-562 using 66829-1-Ig

    1X10^6 K-562 cells were stained with 0.20 ug/test Anti-Human IL12RB1 (66829-1-Ig, Clone:1E10E10) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

Tested Applications

Positive WB detected inRamos cells, TF-1 cells, THP-1 cells, Jurkat cells, Raji cells, K-562 cells, Daudi cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66829-1-Ig targets IL-12RB1 in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-12RB1 fusion protein Ag6920
Full Name interleukin 12 receptor, beta 1
Calculated Molecular Weight 662 aa, 73 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC029121
Gene Symbol IL12RB1
Gene ID (NCBI) 3594
RRIDAB_2882172
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

Protocols

Product Specific Protocols
WB protocol for IL-12RB1 antibody 66829-1-IgDownload protocol
FC protocol for IL-12RB1 antibody 66829-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols