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IL-1F10 Monoclonal antibody

IL-1F10 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

5B2F5

Cat no : 60319-1-Ig

Synonyms

FIL1 theta, FIL1T, FKSG75, IL 1 theta, IL 1F10, IL 1HY2, IL1 theta, IL1F10, IL-1F10, IL1HY2, IL38, Interleukin 1 family member 10, Interleukin 1 HY2, Interleukin 1 theta, UNQ6119/PRO20041

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using IL-1F10 Monoclonal antibody (60319-1-Ig)

    WB analysis of Recombinant protein using 60319-1-Ig

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60319-1-Ig (IL1F10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U-937 cells using IL-1F10 Monoclonal antibody (60319-1-Ig)

    FC experiment of U-937 using 60319-1-Ig

    1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human IL-1F10 (60319-1-Ig, Clone:5B2F5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inRecombinant protein
Positive FC detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60319-1-Ig targets IL-1F10 in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-1F10 fusion protein Ag17250
Full Name interleukin 1 family, member 10 (theta)
Calculated Molecular Weight 152 aa, 17 kDa
GenBank Accession NumberBC103966
Gene Symbol IL1F10
Gene ID (NCBI) 84639
RRIDAB_2881430
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL1F10 is a member of the interleukin 1 cytokine family. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses.

Protocols

Product Specific Protocols
WB protocol for IL-1F10 antibody 60319-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols