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IL-21R Monoclonal antibody

IL-21R Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1D1C2

Cat no : 66319-1-Ig

Synonyms

CD360, IL 21 receptor, IL 21R, IL21R, IL-21R, interleukin 21 receptor, NILR, Novel interleukin receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-21R Monoclonal antibody (66319-1-Ig)

    WB analysis using 66319-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66319-1-Ig (IL-21R antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using IL-21R Monoclonal antibody (66319-1-Ig)

    WB analysis of human placenta using 66319-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66319-1-Ig (IL-21R antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using IL-21R Monoclonal antibody (66319-1-Ig)

    WB analysis of Jurkat using 66319-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66319-1-Ig (IL21R Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-21R Monoclonal antibody (66319-1-Ig)

    IHC staining of human tonsillitis using 66319-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66319-1-Ig (IL21R antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-21R Monoclonal antibody (66319-1-Ig)

    IHC staining of human tonsillitis using 66319-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66319-1-Ig (IL21R antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, human placenta tissue, K-562 cells, TF-1 cells, MOLT-4 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66319-1-Ig targets IL-21R in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IL-21R fusion protein Ag21295
Full Name interleukin 21 receptor
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 55-65 kDa
GenBank Accession NumberBC004348
Gene Symbol IL21R
Gene ID (NCBI) 50615
RRIDAB_2881700
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL21R, a receptor for interleukin-21, belongs to the type I cytokine receptor family. IL21R has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3.

Protocols

Product Specific Protocols
WB protocol for IL-21R antibody 66319-1-IgDownload protocol
IHC protocol for IL-21R antibody 66319-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols