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IL-3 Polyclonal antibody

IL-3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17835-1-AP

Synonyms

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using IL-3 Polyclonal antibody (17835-1-AP)

    WB analysis of Recombinant protein using 17835-1-AP

    Recombinant protein were subjected to SDS PAGE followed by western blot with 17835-1-AP (IL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Recombinant protein using IL-3 Polyclonal antibody (17835-1-AP)

    WB analysis of Recombinant protein using 17835-1-AP

    Recombinant protein were subjected to SDS PAGE followed by western blot with 17835-1-AP (IL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Recombinant protein using IL-3 Polyclonal antibody (17835-1-AP)

    WB analysis of Recombinant protein using 17835-1-AP

    Recombinant protein were subjected to SDS PAGE followed by western blot with 17835-1-AP (IL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-3 Polyclonal antibody (17835-1-AP)

    IHC staining of human tonsillitis using 17835-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17835-1-AP (IL3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-3 Polyclonal antibody (17835-1-AP)

    IHC staining of human tonsillitis using 17835-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17835-1-AP (IL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inRecombinant protein, Recombinant protein protein
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 17835-1-AP is IL-3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivitypig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-3 fusion protein Ag11839
Full Name interleukin 3 (colony-stimulating factor, multiple)
Calculated Molecular Weight 152 aa, 17 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC066273
Gene Symbol IL3
Gene ID (NCBI) 3562
RRIDAB_10644127
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders (PMID: 24103757;2698876;2809196)

Protocols

Product Specific Protocols
WB protocol for IL-3 antibody 17835-1-APDownload protocol
IHC protocol for IL-3 antibody 17835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

JTCVS Open

Intramyocardial injection of hypoxia-conditioned extracellular vesicles modulates apoptotic signaling in chronically ischemic myocardium

Authors - Dwight D Harris
View Article