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CoraLite® Plus 488-conjugated IL-33 Monoclonal antibody

IL-33 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A9C7

Cat no : CL488-66235

Synonyms

C9orf26, DKFZp586H0523, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated IL-33 Monoclonal ant (CL488-66235)

    IF Staining of HepG2 using CL488-66235

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66235 (IL33 antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66235 targets IL-33 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen IL-33 fusion protein Ag21430
Full Name interleukin 33
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC047085
Gene Symbol IL33
Gene ID (NCBI) 90865
RRIDAB_2883291
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodThiophilic affinity chromatograph
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IL-33 antibody CL488-66235Download protocol
Standard Protocols
Click here to view our Standard Protocols