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IL-6R alpha Monoclonal antibody

IL-6R alpha Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A5F4

Cat no : 66855-1-Ig

Synonyms

CD126, gp80, IL 6 receptor subunit alpha, IL 6R 1, IL 6R alpha, IL 6R subunit alpha, IL 6RA, IL6R, IL-6R, IL-6R alpha, IL6RA, interleukin 6 receptor, Membrane glycoprotein 80

Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using IL-6R alpha Monoclonal antibody (66855-1-Ig)

    WB analysis of human spleen using 66855-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66855-1-Ig (IL6R alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood leukocyte using IL-6R alpha Monoclonal antibody (66855-1-Ig)

    WB analysis of human peripheral blood leukocyte using 66855-1-Ig

    Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66855-1-Ig (IL6R alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IL-6R alpha Monoclonal antibody (66855-1-Ig)

    IHC staining of human liver cancer using 66855-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IL-6R alpha Monoclonal antibody (66855-1-Ig)

    IHC staining of human liver cancer using 66855-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-6R alpha Monoclonal antibody (66855-1-Ig)

    IHC staining of human tonsillitis using 66855-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-6R alpha Monoclonal antibody (66855-1-Ig)

    IHC staining of human tonsillitis using 66855-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman spleen tissue, Human peripheral blood leukocyte
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66855-1-Ig targets IL-6R alpha in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-6R alpha fusion protein Ag18263
Full Name interleukin 6 receptor
Calculated Molecular Weight 468 aa, 52 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC132684
Gene Symbol IL6R
Gene ID (NCBI) 3570
RRIDAB_2882195
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).

Protocols

Product Specific Protocols
WB protocol for IL-6R alpha antibody 66855-1-IgDownload protocol
IHC protocol for IL-6R alpha antibody 66855-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols