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ILKAP Polyclonal antibody

ILKAP Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16017-1-AP

Synonyms

ILKAP, PP2C DELTA

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using ILKAP Polyclonal antibody (16017-1-AP)

    WB analysis of human heart using 16017-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16017-1-AP (ILKAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ILKAP Polyclonal antibody (16017-1-AP)

    WB analysis of HepG2 using 16017-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16017-1-AP (ILKAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ILKAP Polyclonal antibody (16017-1-AP)

    WB analysis of K-562 using 16017-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16017-1-AP (ILKAP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using ILKAP Polyclonal antibody (16017-1-AP)

    WB analysis of human heart using 16017-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16017-1-AP (ILKAP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ILKAP Polyclonal antibody (16017-1-AP)

    IHC staining of human stomach cancer using 16017-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16017-1-AP (ILKAP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ILKAP Polyclonal antibody (16017-1-AP)

    IHC staining of human stomach cancer using 16017-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16017-1-AP (ILKAP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman heart tissue, HepG2 cells, K-562 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16017-1-AP targets ILKAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ILKAP fusion protein Ag8826
Full Name integrin-linked kinase-associated serine/threonine phosphatase 2C
Calculated Molecular Weight 392 aa, 43 kDa
Observed Molecular Weight 43-45 kDa
GenBank Accession NumberBC006576
Gene Symbol ILKAP
Gene ID (NCBI) 80895
RRIDAB_2249188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ILKAP antibody 16017-1-APDownload protocol
IHC protocol for ILKAP antibody 16017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols