IMPA1 Polyclonal antibody

IMPA1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16593-1-AP

Synonyms

IMP 1, IMPA, IMPA1, IMPase 1, Inositol monophosphatase 1



Tested Applications

Positive WB detected inrat kidney tissue, human kidney tissue, human brain tissue, mouse kidney tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16593-1-AP targets IMPA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IMPA1 fusion protein Ag9632
Full Name inositol(myo)-1(or 4)-monophosphatase 1
Calculated Molecular Weight 277 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC008381
Gene Symbol IMPA1
Gene ID (NCBI) 3612
RRIDAB_2127216
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IMPA1 is an enzyme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol.

Protocols

Product Specific Protocols
WB protocol for IMPA1 antibody 16593-1-APDownload protocol
IHC protocol for IMPA1 antibody 16593-1-APDownload protocol
IF protocol for IMPA1 antibody 16593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols