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  • KD/KO Validated

ING5 Polyclonal antibody

ING5 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10665-1-AP

Synonyms

ING5, Inhibitor of growth protein 5, p28ING5



Tested Applications

Positive WB detected inJurkat cells, PC-3 cells, mouse kidney tissue, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10665-1-AP targets ING5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ING5 fusion protein Ag1059
Full Name inhibitor of growth family, member 5
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-32 kDa
GenBank Accession NumberBC005370
Gene Symbol ING5
Gene ID (NCBI) 84289
RRIDAB_2280522
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ING5, also named as p28ING5, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 is a component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. (PMID:12750254) Altered ING5 expression and its shift into the cytoplasm may have an impact on the malignant transformation of colorectal epithelial cells and should be considered as a biomarker for colorectal carcinogenesis. (PMID:21193223)

Protocols

Product Specific Protocols
WB protocol for ING5 antibody 10665-1-APDownload protocol
IHC protocol for ING5 antibody 10665-1-APDownload protocol
IP protocol for ING5 antibody 10665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Cell Res

LRP6 downregulation promotes cardiomyocyte proliferation and heart regeneration.

Authors - Yahan Wu
  • KD Validated
mouseWB

EMBO Rep

SCFJFK is functionally linked to obesity and metabolic syndrome.

Authors - Lin He
humanWB

Oncotarget

The miR-193a-3p-regulated ING5 gene activates the DNA damage response pathway and inhibits multi-chemoresistance in bladder cancer.

Authors - Yang Li
humanWB

Oncotarget

ING5 inhibits cancer aggressiveness via preventing EMT and is a potential prognostic biomarker for lung cancer.

Authors - Feng Zhang
humanIF

Oncotarget

ING5 suppresses proliferation, apoptosis, migration and invasion, and induces autophagy and differentiation of gastric cancer cells: a good marker for carcinogenesis and subsequent progression.

Authors - Wen-feng Gou
  • KD Validated
humanWB,IHC

Oncotarget

Upregulated in Hepatitis B virus-associated hepatocellular carcinoma cells, miR-331-3p promotes proliferation of hepatocellular carcinoma cells by targeting ING5.

Authors - Yiyi Cao
  • KD Validated