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INPP5A Polyclonal antibody

INPP5A Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21723-1-AP

Synonyms

5PTASE, INPP5A

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using INPP5A Polyclonal antibody (21723-1-AP)

    WB analysis of HeLa using 21723-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using INPP5A Polyclonal antibody (21723-1-AP)

    WB analysis of HepG2 using 21723-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using INPP5A Polyclonal antibody (21723-1-AP)

    WB analysis of COLO 320 using 21723-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using INPP5A Polyclonal antibody (21723-1-AP)

    WB analysis of human brain using 21723-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, HepG2 cells, human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21723-1-AP is INPP5A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INPP5A fusion protein Ag16380
Full Name inositol polyphosphate-5-phosphatase, 40kDa
Calculated Molecular Weight 412 aa, 48 kDa
Observed Molecular Weight 43-48 kDa
GenBank Accession NumberBC096280
Gene Symbol INPP5A
Gene ID (NCBI) 3632
RRIDAB_10734438
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for INPP5A antibody 21723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dev Cell

Functional genetic screen identifies ITPR3/calcium/RELB axis as a driver of colorectal cancer metastatic liver colonization.

Authors - Ryan H Moy
  • KD Validated
View Article