INPP5K Polyclonal antibody

INPP5K Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15098-1-AP

Synonyms

INPP5K, PPS, SKIP



Tested Applications

Positive WB detected inHEK-293 cells, mouse heart tissue
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15098-1-AP targets INPP5K in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INPP5K fusion protein Ag7192
Full Name inositol polyphosphate-5-phosphatase K
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 45-51 kDa
GenBank Accession NumberBC004362
Gene Symbol INPP5K
Gene ID (NCBI) 51763
RRIDAB_2126365
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for INPP5K antibody 15098-1-APDownload protocol
IHC protocol for INPP5K antibody 15098-1-APDownload protocol
IF protocol for INPP5K antibody 15098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Chem Biol

Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome.

Authors - David Furkert

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tim (Verified Customer) (06-28-2021)

Detected transfected GFP-tagged INPP5K in Cos7 cells well. Antibody incubated with membrane overnight in fridge.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Cos7
INPP5K Antibody Western Blot validation (1/1000 dilution) in Cos7 (Cat no:15098-1-AP)