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- KD/KO Validated
IPO4 Polyclonal antibody
IPO4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 11679-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, human brain tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 9 publications below |
| IHC | See 2 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
11679-1-AP targets IPO4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IPO4 fusion protein Ag2281 |
| Full Name | importin 4 |
| Calculated Molecular Weight | 1081 aa, 118 kDa |
| Observed Molecular Weight | 118 kDa |
| GenBank Accession Number | BC003690 |
| Gene Symbol | IPO4 |
| Gene ID (NCBI) | 79711 |
| RRID | AB_2127677 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Importin‐4 (IPO4) belongs to the importin β family which directs the cargo to pass through the nuclear pore complex via transient interaction with nucleoporins. IPO4 is responsible for transporting histones H3 and H4 into the nucleus for chromatin assembly. Recently IPO4 has been reported to contribute to gastric cancer progression and poor prognosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IPO4 antibody 11679-1-AP | Download protocol |
| IHC protocol for IPO4 antibody 11679-1-AP | Download protocol |
| IF protocol for IPO4 antibody 11679-1-AP | Download protocol |
| IP protocol for IPO4 antibody 11679-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Neddylation of PTEN regulates its nuclear import and promotes tumor development. | ||
Proc Natl Acad Sci U S A CCAAT/enhancer binding protein delta (C/EBPdelta, CEBPD)-mediated nuclear import of FANCD2 by IPO4 augments cellular response to DNA damage. | ||
Proc Natl Acad Sci U S A ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting. | ||
Genomics Proteomics Bioinformatics Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses. | ||
PLoS One An interaction network predicted from public data as a discovery tool: application to the Hsp90 molecular chaperone machine. | ||
