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  • Featured Product
  • KD/KO Validated

IQGAP1 Polyclonal antibody

IQGAP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 22167-1-AP

Synonyms

HUMORFA01, IQGAP1, KIAA0051, p195, SAR1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IQGAP1 Polyclonal antibody (22167-1-AP)

    WB analysis using 22167-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using IQGAP1 Polyclonal antibody (22167-1-AP)

    WB analysis of NIH/3T3 using 22167-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using IQGAP1 Polyclonal antibody (22167-1-AP)

    WB analysis of SGC-7901 using 22167-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using IQGAP1 Polyclonal antibody (22167-1-AP)

    WB analysis of HEK-293 using 22167-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using IQGAP1 Polyclonal antibody (22167-1-AP)

    WB analysis of NIH/3T3 using 22167-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using IQGAP1 Polyclonal antibody (22167-1-AP)

    WB analysis of HeLa using 22167-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using IQGAP1 Polyclonal antibody (22167-1-AP)

    IP experiment of HeLa using 22167-1-AP

    IP result of anti-IQGAP1 (IP:22167-1-AP, 4ug; Detection:22167-1-AP 1:8000) with HeLa cells lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IQGAP1 Polyclonal antibody (22167-1-AP)

    IHC staining of human breast cancer using 22167-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22167-1-AP (IQGAP1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IQGAP1 Polyclonal antibody (22167-1-AP)

    IHC staining of human breast cancer using 22167-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22167-1-AP (IQGAP1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IQGAP1 Polyclonal antibody (22167-1-AP)

    IHC staining of human colon cancer using 22167-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IQGAP1 Polyclonal antibody (22167-1-AP)

    IHC staining of human colon cancer using 22167-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IQGAP1 Polyclonal antibody (22167-1-AP)

    IHC staining of human liver cancer using 22167-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IQGAP1 Polyclonal antibody (22167-1-AP)

    IHC staining of human liver cancer using 22167-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using IQGAP1 Polyclonal antibody (22167-1-PBS)

    IF Staining of A549 using 22167-1-PBS

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using IQGAP1 Polyclonal antibody (22167-1-AP)

    IF Staining of A549 using 22167-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IQGAP1 Polyclonal antibody (22167-1-AP)

    IF Staining of HeLa using 22167-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 22167-1-AP (IQGAP1 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IQGAP1 Polyclonal antibody (22167-1-PBS)

    IF Staining of HeLa using 22167-1-PBS

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using IQGAP1 Polyclonal antibody (22167-1-AP)

    FC experiment of HeLa using 22167-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human IQGAP1 (22167-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA549 cells, NIH/3T3 cells, SGC-7901 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, A549 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22167-1-AP targets IQGAP1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IQGAP1 fusion protein Ag17821
Full Name IQ motif containing GTPase activating protein 1
Calculated Molecular Weight 189 kDa
Observed Molecular Weight 190-195 kDa
GenBank Accession NumberBC151834
Gene Symbol IQGAP1
Gene ID (NCBI) 8826
RRIDAB_10949193
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IQGAP1 is a member of a family of scaffolding proteins that interact with signaling and structural molecules. It localizes to sites of cell-cell contact in epithelial cells and regulates distinct cellular processes including cell adhesion, cell migration, extracellular signals through interacting with numerous protein. Multiple studies have shown that IQGAP1 is up-regulated in many human malignancies, such as lung cancer, ovarian cancer, colon cancer, breast cancer, melanoma and HCC. And IQGAP1 plays a critical role in cancer cell invasion.

Protocols

Product Specific Protocols
WB protocol for IQGAP1 antibody 22167-1-APDownload protocol
IHC protocol for IQGAP1 antibody 22167-1-APDownload protocol
IF protocol for IQGAP1 antibody 22167-1-APDownload protocol
IP protocol for IQGAP1 antibody 22167-1-APDownload protocol
FC protocol for IQGAP1 antibody 22167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Cancer Commun (Lond)

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer

Authors - Xingbin Hu
  • KD Validated
View Article
humanWB

Theranostics

Anti-allergic drug azelastine suppresses colon tumorigenesis by directly targeting ARF1 to inhibit IQGAP1-ERK-Drp1-mediated mitochondrial fission.

Authors - Hui-Fang Hu
View Article
WB

Oncogene

The scaffold protein IQGAP1 links heat-induced stress signals to alternative splicing regulation in gastric cancer cells.

Authors - Andrada-Maria Birladeanu
  • KO Validated
View Article
humanWB

Oncotarget

Diet-induced hypercholesterolemia promotes androgen-independent prostate cancer metastasis via IQGAP1 and caveolin-1.

Authors - Hyeongsun Moon
View Article
humanWB,IF

Front Cell Infect Microbiol

IQGAP1 Mediates Hcp1-Promoted Escherichia coli Meningitis by Stimulating the MAPK Pathway.

Authors - Mingna Zhao
  • KD Validated
View Article
humanIHC

BMC Cancer

Comprehensive analysis of the expression and prognosis for IQ motif-containing GTPase-activating proteins in hepatocellular carcinoma

Authors - Qingqing Dai
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Joleen (Verified Customer) (06-16-2019)

The antibody recognizes IQGAP (top band) but also another band at a lower molecular weight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
IQGAP1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:22167-1-AP)
FH

Chun-Chih (Verified Customer) (01-31-2019)

Works well in IP at recommended conc. Clear and strong band, 1mg protein lysate per condition.

  • Applications: Immunoprecipitation,
  • Cell Tissue Type: Human breast cancer cells