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ITCH Monoclonal antibody

ITCH Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E3G9

Cat no : 67757-1-Ig

Synonyms

AIF4, AIP4, dJ468O1.1, ITCH, NAPP1, NFE2 associated polypeptide 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ITCH Monoclonal antibody (67757-1-Ig)

    WB analysis using 67757-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ITCH Monoclonal antibody (67757-1-Ig)

    WB analysis of K-562 using 67757-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using ITCH Monoclonal antibody (67757-1-Ig)

    WB analysis of HSC-T6 using 67757-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using ITCH Monoclonal antibody (67757-1-Ig)

    WB analysis of LNCaP using 67757-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using ITCH Monoclonal antibody (67757-1-Ig)

    WB analysis of NIH/3T3 using 67757-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ITCH Monoclonal antibody (67757-1-Ig)

    IF Staining of HepG2 using 67757-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ITCH antibody (67757-1-Ig, Clone: 2E3G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, K-562 cells, LNCaP cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67757-1-Ig targets ITCH in WB, IP, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ITCH fusion protein Ag14688
Full Name itchy E3 ubiquitin protein ligase homolog (mouse)
Calculated Molecular Weight 903 aa, 103 kDa
Observed Molecular Weight103 kDa, 70 kDa
GenBank Accession NumberBC011571
Gene Symbol ITCH
Gene ID (NCBI) 83737
RRIDAB_2918525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ITCH antibody 67757-1-IgDownload protocol
IF protocol for ITCH antibody 67757-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Biomedicines

ITCH-Mediated Ubiquitylation of ITGB3 Promotes Cell Proliferation and Invasion of Ectopic Endometrial Stromal Cells in Ovarian Endometriosis

Authors - Liansuo Zhang
View Article