ITLN1/2 Polyclonal antibody

ITLN1/2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11770-1-AP

Synonyms

Endothelial lectin HL 1, Galactofuranose binding lectin, hIntL, HL 1, HL1, Intelectin 1, INTL, ITLN, ITLN 1, ITLN1, ITLN2, LFR, omentin



Tested Applications

Positive WB detected inmouse colon tissue
Positive IP detected inmouse appendix tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11770-1-AP targets ITLN1/2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ITLN1/2 fusion protein Ag2424
Full Name intelectin 1 (galactofuranose binding)
Calculated Molecular Weight 313 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC020664
Gene Symbol ITLN1
Gene ID (NCBI) 55600
RRIDAB_2129677
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITLN1, also named as Intelectin 1, has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. ITLN1 is a glycoprotein made up of three 40 kDa subunits cross-linked by disulfide bonds making up a 120-kDa homo-trimer of 295 amino acids and N-linked oligosaccharides (PMID: 17621593). It may play a role in the defense system against microorganisms. Human intelectin-1 is expressed in intestinal goblet cells, and is present at about 200 ng/mL in serum. Intelectin-1 was also shown to be expressed in human endothelial cells and adipocytes of human abdominal adipose tissue. (PMID: 22768319,PMID: 28640441)

Protocols

Product Specific Protocols
WB protocol for ITLN1/2 antibody 11770-1-APDownload protocol
IP protocol for ITLN1/2 antibody 11770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Nat Commun

Tissue mechanics drives regeneration of a mucociliated epidermis on the surface of Xenopus embryonic aggregates.

Authors - Hye Young Kim
humanIHC, IF

J Exp Med

Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.

Authors - Yalong Wang
mouseWB,IHC

Mucosal Immunol

Intelectin contributes to allergen-induced IL-25, IL-33, and TSLP expression and type 2 response in asthma and atopic dermatitis.

Authors - L Yi
mouseWB,IF

Front Cell Dev Biol

Neuroprotective Effects of Omentin-1 Against Cerebral Hypoxia/Reoxygenation Injury via Activating GAS6/Axl Signaling Pathway in Neuroblastoma Cells.

Authors - Xiaochen Niu
humanWB

Fish Shellfish Immunol

Molecular properties and ligand specificity of zebrafish intelectin-2.

Authors - Nongnuch Singrang
humanWB

J Biol Chem

Structures of Xenopus embryonic epidermal lectin reveal a conserved mechanism of microbial glycan recognition.

Authors - Kittikhun Wangkanont