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JPH1 Polyclonal antibody

JPH1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 25979-1-AP

Synonyms

JP 1, JP1, JPH1, junctophilin 1, Junctophilin type 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using JPH1 Polyclonal antibody (25979-1-AP)

    WB analysis of HEK-293 using 25979-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25979-1-AP (JPH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using JPH1 Polyclonal antibody (25979-1-AP)

    WB analysis using 25979-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25979-1-AP (JPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using JPH1 Polyclonal antibody (25979-1-AP)

    IHC staining of human skeletal muscle using 25979-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using JPH1 Polyclonal antibody (25979-1-AP)

    IHC staining of human skeletal muscle using 25979-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using JPH1 Polyclonal antibody (25979-1-AP)

    IHC staining of human skin using 25979-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using JPH1 Polyclonal antibody (25979-1-AP)

    IHC staining of human skin using 25979-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25979-1-AP (JPH1 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman skeletal muscle tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25979-1-AP is JPH1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JPH1 fusion protein Ag23270
Full Name junctophilin 1
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC140875
Gene Symbol JPH1
Gene ID (NCBI) 56704
RRIDAB_2880317
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Junctophilin-1 is a protein that in humans is encoded by the JPH1 gene. This gene is a member of the junctophilin gene family. Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane.

Protocols

Product Specific Protocols
WB protocol for JPH1 antibody 25979-1-APDownload protocol
IHC protocol for JPH1 antibody 25979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols