KANK2 Polyclonal antibody
KANK2 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 21733-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, mouse kidney tissue, Transfected HEK-293 cells, mouse lung tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
21733-1-AP targets KANK2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KANK2 fusion protein Ag16416 |
| Full Name | KN motif and ankyrin repeat domains 2 |
| Calculated Molecular Weight | 851 aa, 91 kDa |
| Observed Molecular Weight | 95-100 kDa |
| GenBank Accession Number | BC098105 |
| Gene Symbol | KANK2 |
| Gene ID (NCBI) | 25959 |
| RRID | AB_10732728 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Involved in transcription regulation by sequestering nuclear receptor coactivators, such as NCOA1, NCOA2 and NCOA3, in the cytoplasm; the function is deregulated by phosphorylation. May be involved in the control of cytoskeleton formation by regulating actin polymerization. Involved in regulation of caspase-independent apoptosis; proposed to sequester AIFM1 in mitochondria and apoptotic stimuli lead to its proteasomal degradation allowing the release of AIFM1 to the nucleus. May be involved in promotion of cell proliferation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KANK2 antibody 21733-1-AP | Download protocol |
| IHC protocol for KANK2 antibody 21733-1-AP | Download protocol |
| IF protocol for KANK2 antibody 21733-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol The RNA-binding protein Fus directs translation of localized mRNAs in APC-RNP granules. | ||
J Biol Chem Nephrotic syndrome-associated mutation of KANK2 induces pathologic binding competition with physiological interactor KIF21A. | ||
Am J Physiol Cell Physiol Knockout of the neonatal Fc Receptor (FcRn) in cultured podocytes alters IL-6 signaling and the actin cytoskeleton. | ||
Am J Physiol Cell Physiol Itga8-Cre-mediated deletion of YAP and TAZ impairs bladder contractility with minimal inflammation and chondrogenic differentiation |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Bryce (Verified Customer) (09-12-2022) | Strong band at expected molecular weight.
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