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  • Featured Product
  • KD/KO Validated

Kv1.3 Polyclonal antibody

Kv1.3 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 14079-1-AP

Synonyms

HGK5, HLK3, HPCN3, HUKIII, KCNA3, Kv1.3, MK3, PCN3

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using Kv1.3 Polyclonal antibody (14079-1-AP)

    WB analysis of A549 using 14079-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14079-1-AP (Kv1.3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Kv1.3 Polyclonal antibody (14079-1-AP)

    WB analysis of A549 using 14079-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14079-1-AP (Kv1.3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using Kv1.3 Polyclonal antibody (14079-1-AP)

    WB analysis of mouse thymus using 14079-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14079-1-AP (Kv1.3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Rat brain tissue using Kv1.3 Polyclonal antibody (14079-1-AP)

    IF Staining of Rat brain using 14079-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Rat brain tissue using Kv1.3 antibody (14079-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, mouse thymus tissue
Positive IF detected inRat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14079-1-AP targets Kv1.3 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kv1.3 fusion protein Ag5204
Full Name potassium voltage-gated channel, shaker-related subfamily, member 3
Calculated Molecular Weight 575 aa, 64 kDa
Observed Molecular Weight 52 kDa, 75 kDa
GenBank Accession NumberBC035059
Gene Symbol KCNA3
Gene ID (NCBI) 3738
RRIDAB_2878011
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kv1.3 ( also known as KCNA3, MK3, HGK5, HLK3, PCN3, HPCN3) contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. It plays an essential role in T cell proliferation and activation. Kv1.3 channels are expressed in in various tissues and cell types including brain, vascular smooth muscle cells and leucocytes. Malfunction of Kv1.3 can alter the immune response, elicit neurotoxic effects or impact on cancer growth(PMID: 24305723; 24133455). 14079-1-AP can detect two isoforms of Kv1.3 other than the canonical channel (60 KDa) : A longer isoform (70 KDa) and a truncated isoform (43 KDa).

Protocols

Product Specific Protocols
WB protocol for Kv1.3 antibody 14079-1-APDownload protocol
IF protocol for Kv1.3 antibody 14079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Mol Immunol

NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology.

Authors - Xiaolong He
  • KD Validated
View Article
humanWB

FASEB J

The Kv1.3 ion channel acts as a host factor restricting viral entry.

Authors - Yange Lang
View Article
ratWB

Int J Clin Exp Med

Inhibition of 15-lipoxygenase (15-LOX) reverses hypoxia-induced down-regulation of potassium channels Kv1.5 and Kv2.1Inhibition of 15-lipoxygenase (15-LOX) reverses hypoxia-induced down-regulation of potassium channels Kv1.5 and Kv2.1.

Authors - Wenjuan Liu
View Article