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KCNH7 Polyclonal antibody

KCNH7 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 13622-1-AP

Synonyms

Eag related protein 3, ERG 3, ERG3, hERG 3, HERG3, KCNH7, Kv11.3

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using KCNH7 Polyclonal antibody (13622-1-AP)

    WB analysis of human brain using 13622-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13622-1-AP (KCNH7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using KCNH7 Polyclonal antibody (13622-1-AP)

    WB analysis of mouse brain using 13622-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13622-1-AP (KCNH7 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KCNH7 Polyclonal antibody (13622-1-AP)

    IP experiment of mouse brain using 13622-1-AP

    IP result of anti-KCNH7 (IP:13622-1-AP, 4ug; Detection:13622-1-AP 1:300) with mouse brain tissue lysate 5200ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using KCNH7 Polyclonal antibody (13622-1-AP)

    IHC staining of human heart using 13622-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 13622-1-AP (KCNH7 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using KCNH7 Polyclonal antibody (13622-1-AP)

    IHC staining of human heart using 13622-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 13622-1-AP (KCNH7 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13622-1-AP targets KCNH7 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNH7 fusion protein Ag4532
Full Name potassium voltage-gated channel, subfamily H (eag-related), member 7
Calculated Molecular Weight 1196 aa, 135 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC035815
Gene Symbol KCNH7
Gene ID (NCBI) 90134
RRIDAB_10638620
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KCNH7 antibody 13622-1-APDownload protocol
IHC protocol for KCNH7 antibody 13622-1-APDownload protocol
IP protocol for KCNH7 antibody 13622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols