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KCNJ3 Polyclonal antibody

KCNJ3 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 16985-1-AP

Synonyms

GIRK 1, GIRK1, KCNJ3, KGA, KIR3.1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using KCNJ3 Polyclonal antibody (16985-1-AP)

    WB analysis of mouse brain using 16985-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16985-1-AP (KCNJ3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KCNJ3 Polyclonal antibody (16985-1-AP)

    IF Staining of HeLa using 16985-1-AP

    Immunofluorescent analysis of HeLa cells using 16985-1-AP (KCNJ3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inmouse brain tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 16985-1-AP is KCNJ3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNJ3 fusion protein Ag10581
Full Name potassium inwardly-rectifying channel, subfamily J, member 3
Calculated Molecular Weight 501 aa, 57 kDa
GenBank Accession NumberBC022495
Gene Symbol KCNJ3
Gene ID (NCBI) 3760
RRIDAB_2878336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KCNJ3 antibody 16985-1-APDownload protocol
IF protocol for KCNJ3 antibody 16985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols