GIRK2 Polyclonal antibody

GIRK2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21647-1-AP

Synonyms

BIR1, GIRK 2, GIRK2, hiGIRK2, KATP 2, KATP2, KCNJ6, KCNJ7, KIR3.2



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21647-1-AP is GIRK2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GIRK2 fusion protein Ag16344
Full Name potassium inwardly-rectifying channel, subfamily J, member 6
Calculated Molecular Weight 423 aa, 48 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC101547
Gene Symbol KCNJ6
Gene ID (NCBI) 3763
RRIDAB_10794447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GIRK2 (also known as Kir3.2), encoded by KCNJ6 gene, is a member of the G protein-coupled inwardly-rectifying potassium channel (GIRK, Kir3) family of inward rectifier potassium channels. GIRK channels are activated following stimulation of G protein-coupled receptors (PMID: 26422984). They play important roles in regulating cellular excitabilities in the heart and brain (PMID: 31043612). Mutations in KCNJ6 gene are associated with Keppen-Lubinsky Syndrome (PMID: 25620207).

Protocols

Product Specific Protocols
WB protocol for GIRK2 antibody 21647-1-APDownload protocol
IHC protocol for GIRK2 antibody 21647-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Ther Nucleic Acids

Modulation of miR-181 influences dopaminergic neuronal degeneration in a mouse model of Parkinson's disease.

Authors - Colleen S Stein
mouseWB

FASEB J

Electro-acupuncture alleviates adolescent cocaine exposure-enhanced anxiety-like behaviors in adult mice by attenuating the activities of PV interneurons in PrL.

Authors - Jiaxun Nie
humanIF

Front Neurosci

Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease

Authors - Fahad Mubariz

Prog Neurobiol

TRPM2 as a conserved gatekeeper determines the vulnerability of DA neurons by mediating ROS sensing and calcium dyshomeostasis

Authors - Peiwu Ye