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  • Featured Product
  • KD/KO Validated

KCTD12 Polyclonal antibody

KCTD12 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15523-1-AP

Synonyms

C13orf2, KCTD12, KIAA1778, PFET1, PFETIN

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using KCTD12 Polyclonal antibody (15523-1-AP)

    WB analysis of mouse brain using 15523-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KCTD12 Polyclonal antibody (15523-1-AP)

    WB analysis of HeLa using 15523-1-AP

    Hela cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using KCTD12 Polyclonal antibody (15523-1-AP)

    WB analysis of mouse brain using 15523-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using KCTD12 Polyclonal antibody (15523-1-AP)

    WB analysis of MCF-7 using 15523-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using KCTD12 Polyclonal antibody (15523-1-AP)

    WB analysis of rat brain using 15523-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using KCTD12 Polyclonal antibody (15523-1-AP)

    WB analysis of human brain using 15523-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using KCTD12 Polyclonal antibody (15523-1-AP)

    WB analysis of HEK-293 using 15523-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KCTD12 Polyclonal antibody (15523-1-AP)

    IF Staining of HeLa using 15523-1-AP

    Immunofluorescent analysis of Hela cells, using KCTD12 antibody 15523-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse brain tissue, MCF-7 cells, human brain tissue, HEK-293 cells, Hela cells, rat brain tissue
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15523-1-AP targets KCTD12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCTD12 fusion protein Ag5716
Full Name potassium channel tetramerisation domain containing 12
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC013764
Gene Symbol KCTD12
Gene ID (NCBI) 115207
RRIDAB_2132006
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KCTD12 antibody 15523-1-APDownload protocol
IF protocol for KCTD12 antibody 15523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Oncogene

KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition.

Authors - Y Zhong
  • KD Validated
View Article
mouseWB,IF

Pharmacol Res

The effects of Kctd12, an auxiliary subunit of GABAB receptor in dentate gyrus on behavioral response to chronic social defeat stress in mice.

Authors - Si-Long Deng
View Article
mouseWB,IF

J Neurosci

KCTD8 and KCTD12 Facilitate Axonal Expression of GABAB Receptors in Habenula Cholinergic Neurons.

Authors - Yuqi Ren
  • KO Validated
View Article
humanWB,IHC

Sci Rep

KCTD12 Regulates Colorectal Cancer Cell Stemness through the ERK Pathway.

Authors - Liping Li
  • KD Validated
View Article
mouseIF

Front Mol Neurosci

The Electrophysiological Determinants of Corticospinal Motor Neuron Vulnerability in ALS.

Authors - Javier H Jara
View Article
mouseWB

PLoS One

Mice lacking the transcriptional regulator Bhlhe40 have enhanced neuronal excitability and impaired synaptic plasticity in the hippocampus.

Authors - Kelly A Hamilton
View Article