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KIAA1468 Polyclonal antibody

KIAA1468 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18993-1-AP

Synonyms

HsT3308, HsT885, KIAA1468, RAB11-binding protein RELCH, RELCH

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA1468 Polyclonal antibody (18993-1-AP)

    WB analysis using 18993-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18993-1-AP (KIAA1468 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KIAA1468 Polyclonal antibody (18993-1-AP)

    IP experiment of mouse brain using 18993-1-AP

    IP result of anti-KIAA1468 (IP:18993-1-AP, 4ug; Detection:18993-1-AP 1:500) with mouse brain tissue lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using KIAA1468 Polyclonal antibody (18993-1-AP)

    IHC staining of mouse kidney using 18993-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18993-1-AP (KIAA1468 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using KIAA1468 Polyclonal antibody (18993-1-AP)

    IHC staining of mouse kidney using 18993-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18993-1-AP (KIAA1468 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18993-1-AP targets KIAA1468 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name KIAA1468
Calculated Molecular Weight 139 kDa
Observed Molecular Weight 100-135 kDa
GenBank Accession NumberNM_020854
Gene Symbol KIAA1468
Gene ID (NCBI) 57614
RRIDAB_10598164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RELCH/KIAA1468, which is a newly identified Rab11 effector protein, tethers the recycling endosomes (REs) and trans-Golgi network (TGN) membranes by binding TGN-localized oxysterol-binding protein (OSBP) and promotes OSBP-dependent nonvesicular cholesterol transport from REs to the TGN.

Protocols

Product Specific Protocols
WB protocol for KIAA1468 antibody 18993-1-APDownload protocol
IHC protocol for KIAA1468 antibody 18993-1-APDownload protocol
IP protocol for KIAA1468 antibody 18993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols