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  • Featured Product
  • KD/KO Validated

KIF22 Polyclonal antibody

KIF22 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13403-1-AP

Synonyms

KID, KIF22, kinesin family member 22, Kinesin like protein 4, Kinesin like protein KIF22, KNSL4, OBP, OBP 1, OBP 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIF22 Polyclonal antibody (13403-1-AP)

    WB analysis using 13403-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13403-1-AP (KIF22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using KIF22 Polyclonal antibody (13403-1-AP)

    WB analysis of human heart using 13403-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13403-1-AP (KIF22 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using KIF22 Polyclonal antibody (13403-1-AP)

    IP experiment of HeLa using 13403-1-AP

    IP result of anti-KIF22 (IP:13403-1-AP, 4ug; Detection:13403-1-AP 1:300) with HeLa cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using KIF22 Polyclonal antibody (13403-1-AP)

    IHC staining of human cervical cancer using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13403-1-AP (KIF22 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using KIF22 Polyclonal antibody (13403-1-AP)

    IHC staining of human cervical cancer using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13403-1-AP (KIF22 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KIF22 Polyclonal antibody (13403-1-AP)

    IHC staining of human breast cancer using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13403-1-AP (KIF22 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KIF22 Polyclonal antibody (13403-1-AP)

    IHC staining of human breast cancer using 13403-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13403-1-AP (KIF22 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, human heart tissue, MKN-45 cells, T-47D cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13403-1-AP targets KIF22 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF22 fusion protein Ag4254
Full Name kinesin family member 22
Calculated Molecular Weight 665 aa, 73 kDa
Observed Molecular Weight 68-73 kDa
GenBank Accession NumberBC028155
Gene Symbol KIF22
Gene ID (NCBI) 3835
RRIDAB_2249576
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF22, also known as KID (kinesin-like DNA-binding protein), is a kinesin-like DNA-binding protein known to be involved in chromosome movement during mitosis. KIF22 is a nuclear protein regulated by SIAH-1, whose level fluctuate in a cell cycle-dependent manner, increasing during pre-mitotic phases and greatly decreasing during mitosis. KIF22 localizes to the nucleus of the interphase cells upon entry into mitosis and displays some punctate cytoplasmic staining. Defects in KIF22 are the cause of spondyloepimetaphyseal dysplasia with joint laxity, type 2 (SEMDJL2).

Protocols

Product Specific Protocols
WB protocol for KIF22 antibody 13403-1-APDownload protocol
IHC protocol for KIF22 antibody 13403-1-APDownload protocol
IP protocol for KIF22 antibody 13403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Sci Rep

PMID: 29229923

Authors - Sara Bizzotto
View Article
humanWB

Proteomics

Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A, a proteomics study.

Authors - Yan Guang-Rong GR
View Article
humanWB,IHC

Chin Med J (Engl)

Effect of KIF22 on promoting proliferation and migration of gastric cancer cells via MAPK-ERK pathways.

Authors - Ze-Yuan Yu
  • KD Validated
View Article