• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

KIN17 Polyclonal antibody

KIN17 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 12313-1-AP

Synonyms

Binding to curved DNA, BTCD, DNA/RNA binding protein KIN17, KIN, KIN17

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using KIN17 Polyclonal antibody (12313-1-AP)

    WB analysis of HeLa using 12313-1-AP

    HeLa cells, mouse testis tissue, and rat testis tissue were subjected to SDS PAGE followed by western blot with 12313-1-AP (KIN17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using KIN17 Polyclonal antibody (12313-1-AP)

    WB analysis of human heart using 12313-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12313-1-AP (KIN17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KIN17 Polyclonal antibody (12313-1-AP)

    WB analysis of HeLa using 12313-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12313-1-AP (KIN17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KIN17 Polyclonal antibody (12313-1-AP)

    IP experiment of mouse brain using 12313-1-AP

    IP result of anti-KIN17 (IP:12313-1-AP, 4ug; Detection:12313-1-AP 1:500) with mouse brain tissue lysate 3160 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KIN17 Polyclonal antibody (12313-1-AP)

    IF Staining of HeLa using 12313-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12313-1-AP (KIN17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, human heart tissue
Positive IP detected inmouse brain tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12313-1-AP targets KIN17 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIN17 fusion protein Ag2963
Full Name KIN, antigenic determinant of recA protein homolog (mouse)
Calculated Molecular Weight 393 aa, 45 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC017309
Gene Symbol KIN
Gene ID (NCBI) 22944
RRIDAB_2130343
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIN17 (also known as KIN) is an evolutionarily conserved zinc finger nuclear protein. It consists of three motifs, a zinc finger, a nuclear localization signal and a Kyprides-Onzonis-Woese motif that mediates transcription elongation. KIN17 is ubiquitously expressed in human tissues and participates in DNA replication, DNA repair and cell cycle progression. Emerging evidence has indicated the importance of KIN17 in the pathogenesis and progression of tumors (PMID: 28454391). KIN17 has a calculated molecular wight of 45 kDa, this antibody is raised against human KIN17 and detects a band of about 40 kDa.

Protocols

Product Specific Protocols
WB protocol for KIN17 antibody 12313-1-APDownload protocol
IF protocol for KIN17 antibody 12313-1-APDownload protocol
IP protocol for KIN17 antibody 12313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols