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KISS1R-Specific Polyclonal antibody

KISS1R-Specific Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15505-1-AP

Synonyms

AXOR12, G protein coupled receptor 54, GPR54, HOT7T175, Hypogonadotropin 1, KiSS 1 receptor, KiSS 1R, KISS1 receptor, KISS1R, KISS1R-Specific, Kisspeptins receptor, Metastin receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    WB analysis of mouse cerebellum using 15505-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    WB analysis of SH-SY5Y using 15505-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    WB analysis of MCF-7 using 15505-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    IHC staining of mouse cerebellum using 15505-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    IHC staining of mouse cerebellum using 15505-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    IHC staining of mouse cerebellum using 15505-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    IHC staining of mouse cerebellum using 15505-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15505-1-AP (KISS1R-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    IF Staining of MCF-7 using 15505-1-AP

    Immunofluorescent analysis of MCF-7 cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    IF Staining of HeLa using 15505-1-AP

    Immunofluorescent analysis of Hela cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    IF Staining of HepG2 using 15505-1-AP

    Immunofluorescent analysis of HepG2 cells, using KISS1R antibody 15505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using KISS1R-Specific Polyclonal antibody (15505-1-AP)

    FC experiment of SH-SY5Y using 15505-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug KISS1R-Specific antibody (15505-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inmouse cerebellum tissue, MCF-7 cells, SH-SY5Y cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, Hela cells, HepG2 cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15505-1-AP targets KISS1R-Specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name KISS1 receptor
Calculated Molecular Weight 43 kDa
Observed Molecular Weight75 kDa, 43-45 kDa
GenBank Accession NumberNM_032551
Gene Symbol KISS1R
Gene ID (NCBI) 84634
RRIDAB_10640578
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KISS1R is the receptor for metastin (kisspeptin-54, 13, 14, 10), C- terminally amidated peptides of KISS-1. KISS-1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some other carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.

Protocols

Product Specific Protocols
WB protocol for KISS1R-Specific antibody 15505-1-APDownload protocol
IHC protocol for KISS1R-Specific antibody 15505-1-APDownload protocol
IF protocol for KISS1R-Specific antibody 15505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Cell Physiol

Kp-10 promotes bovine mammary epithelial cell proliferation by activating GPR54 and its downstream signaling pathways.

Authors - Yanwei Li
View Article
bovineWB,IF

J Agric Food Chem

Kisspeptin-10 Maintains the Activation of the mTOR Signaling Pathway by Inhibiting SIRT6 to Promote the Synthesis of Milk in Bovine Mammary Epithelial Cells.

Authors - Yu Cao
View Article
bovineWB

Int J Mol Sci

Kisspeptin-10 Induces β-Casein Synthesis via GPR54 and Its Downstream Signaling Pathways in Bovine Mammary Epithelial Cells.

Authors - Jianhua Sun
View Article
humanWB

Mol Oncol

Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis

Authors - Tianwei Chen
View Article