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  • Featured Product
  • KD/KO Validated

KLC1 Polyclonal antibody

KLC1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19028-1-AP

Synonyms

kinesin light chain 1, KLC, KLC 1, KLC1, KNS2, KNS2A

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using KLC1 Polyclonal antibody (19028-1-AP)

    WB analysis of mouse brain using 19028-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using KLC1 Polyclonal antibody (19028-1-AP)

    WB analysis of COLO 320 using 19028-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using KLC1 Polyclonal antibody (19028-1-AP)

    WB analysis of SH-SY5Y using 19028-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using KLC1 Polyclonal antibody (19028-1-AP)

    WB analysis of HepG2 using 19028-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19028-1-AP (KLC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using KLC1 Polyclonal antibody (19028-1-AP)

    IHC staining of human brain using 19028-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19028-1-AP (KLC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using KLC1 Polyclonal antibody (19028-1-AP)

    IHC staining of human brain using 19028-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19028-1-AP (KLC1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KLC1 Polyclonal antibody (19028-1-AP)

    IF Staining of HeLa using 19028-1-AP

    Immunofluorescent analysis of Hela cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KLC1 Polyclonal antibody (19028-1-AP)

    IF Staining of HeLa using 19028-1-AP

    Immunofluorescent analysis of Hela cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KLC1 Polyclonal antibody (19028-1-AP)

    IF Staining of HepG2 using 19028-1-AP

    Immunofluorescent analysis of HepG2 cells, using KLC1 antibody 19028-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inmouse brain tissue, COLO 320 cells, HepG2 cells, SH-SY5Y cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19028-1-AP is KLC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLC1 fusion protein Ag13570
Full Name kinesin light chain 1
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 61-70 kDa
GenBank Accession NumberBC008881
Gene Symbol KLC1
Gene ID (NCBI) 3831
RRIDAB_10641041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KLC1 antibody 19028-1-APDownload protocol
IHC protocol for KLC1 antibody 19028-1-APDownload protocol
IF protocol for KLC1 antibody 19028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FASEB J

Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.

Authors - Amélie Robert
  • KO Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Boyan (Verified Customer) (09-28-2020)

This antibody recognised the expected bands just like what is shown in its technical paper.

  • Applications: Western Blot